mixed milt fertilization of endangered caspian brown trout salmo trutta caspius influences effective population size of breeders

the maintenance of the endangered caspian brown trout salmo trutta caspius populations in iran depends on its artificial breeding. there is no information on how current hatchery breeding protocol influences effective population size of breeders (ne), which is a critical parameter to maintain genetic diversity in future generations. the current hatchery protocol (chp) was comparatively evaluated with a balanced hatchery protocol (bhp), where mixtures of equal amounts of gametes per male and female breeder were used to balance parental contributions to progeny. to evaluate putative differences in viability between families, individual crosses were performed and fertilized ova of different families were mixed to constitute balanced family pools. 1440 alevins were totally sampled in the crosses performed from the 11 breeders. an exclusion-based parentage approach using three polymorphic microsatellite markers unambiguously assigned more than 93% of progeny to a single pair of parents. significantly different contributions of breeders to progeny were observed in chp (p